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human interleukin 1β  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec human interleukin 1β
    Human Interleukin 1β, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/interleukin/Human+IL-1%CE%B2%2C+premium+grade/pm42478839-454-20-22
    Average 94 stars, based on 28 article reviews
    human interleukin 1β - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    In Vitro:

    Article Title: Preclinical targeting of human acute myeloid leukemia and myeloablation using chimeric antigen receptor–modified T cells
    Article Snippet: The pELNS anti-CD123-41BB-CD3ζ (CAR123) plasmid DNA was similarly generated by cloning light-to-heavy or heavy-to-light chain orientations of the mouse anti-human CD123 scFv (clone 32716 or clone 26292 28 ), custom synthesized by Geneart, into the CAR19 construct. .. Normal donor T cells were positively selected from leukapheresis packs using anti-CD4 and CD8 microbeads (Miltenyi), expanded in vitro with anti-CD3/CD28 beads (Invitrogen) and interleukin-2 100 U/mL (Chiron) for up to 15 days, and transduced with lentiviral supernatant from 293T cells transfected with pELNS anti-CD123-41BB-CD3ζ plasmid DNA or with pELNS anti-CD19-41BB-CD3ζ plasmid DNA as previously reported 27 beginning on day +1 of expansion at a multiplicity of infection of 5. .. Cells The MOLM14 cell line was obtained from the ATCC and maintained in RPMI media supplemented with 10% fetal calf serum, penicillin, and streptomycin (R10).

    Transduction:

    Article Title: Preclinical targeting of human acute myeloid leukemia and myeloablation using chimeric antigen receptor–modified T cells
    Article Snippet: The pELNS anti-CD123-41BB-CD3ζ (CAR123) plasmid DNA was similarly generated by cloning light-to-heavy or heavy-to-light chain orientations of the mouse anti-human CD123 scFv (clone 32716 or clone 26292 28 ), custom synthesized by Geneart, into the CAR19 construct. .. Normal donor T cells were positively selected from leukapheresis packs using anti-CD4 and CD8 microbeads (Miltenyi), expanded in vitro with anti-CD3/CD28 beads (Invitrogen) and interleukin-2 100 U/mL (Chiron) for up to 15 days, and transduced with lentiviral supernatant from 293T cells transfected with pELNS anti-CD123-41BB-CD3ζ plasmid DNA or with pELNS anti-CD19-41BB-CD3ζ plasmid DNA as previously reported 27 beginning on day +1 of expansion at a multiplicity of infection of 5. .. Cells The MOLM14 cell line was obtained from the ATCC and maintained in RPMI media supplemented with 10% fetal calf serum, penicillin, and streptomycin (R10).

    Transfection:

    Article Title: Preclinical targeting of human acute myeloid leukemia and myeloablation using chimeric antigen receptor–modified T cells
    Article Snippet: The pELNS anti-CD123-41BB-CD3ζ (CAR123) plasmid DNA was similarly generated by cloning light-to-heavy or heavy-to-light chain orientations of the mouse anti-human CD123 scFv (clone 32716 or clone 26292 28 ), custom synthesized by Geneart, into the CAR19 construct. .. Normal donor T cells were positively selected from leukapheresis packs using anti-CD4 and CD8 microbeads (Miltenyi), expanded in vitro with anti-CD3/CD28 beads (Invitrogen) and interleukin-2 100 U/mL (Chiron) for up to 15 days, and transduced with lentiviral supernatant from 293T cells transfected with pELNS anti-CD123-41BB-CD3ζ plasmid DNA or with pELNS anti-CD19-41BB-CD3ζ plasmid DNA as previously reported 27 beginning on day +1 of expansion at a multiplicity of infection of 5. .. Cells The MOLM14 cell line was obtained from the ATCC and maintained in RPMI media supplemented with 10% fetal calf serum, penicillin, and streptomycin (R10).

    Plasmid Preparation:

    Article Title: Preclinical targeting of human acute myeloid leukemia and myeloablation using chimeric antigen receptor–modified T cells
    Article Snippet: The pELNS anti-CD123-41BB-CD3ζ (CAR123) plasmid DNA was similarly generated by cloning light-to-heavy or heavy-to-light chain orientations of the mouse anti-human CD123 scFv (clone 32716 or clone 26292 28 ), custom synthesized by Geneart, into the CAR19 construct. .. Normal donor T cells were positively selected from leukapheresis packs using anti-CD4 and CD8 microbeads (Miltenyi), expanded in vitro with anti-CD3/CD28 beads (Invitrogen) and interleukin-2 100 U/mL (Chiron) for up to 15 days, and transduced with lentiviral supernatant from 293T cells transfected with pELNS anti-CD123-41BB-CD3ζ plasmid DNA or with pELNS anti-CD19-41BB-CD3ζ plasmid DNA as previously reported 27 beginning on day +1 of expansion at a multiplicity of infection of 5. .. Cells The MOLM14 cell line was obtained from the ATCC and maintained in RPMI media supplemented with 10% fetal calf serum, penicillin, and streptomycin (R10).

    Infection:

    Article Title: Preclinical targeting of human acute myeloid leukemia and myeloablation using chimeric antigen receptor–modified T cells
    Article Snippet: The pELNS anti-CD123-41BB-CD3ζ (CAR123) plasmid DNA was similarly generated by cloning light-to-heavy or heavy-to-light chain orientations of the mouse anti-human CD123 scFv (clone 32716 or clone 26292 28 ), custom synthesized by Geneart, into the CAR19 construct. .. Normal donor T cells were positively selected from leukapheresis packs using anti-CD4 and CD8 microbeads (Miltenyi), expanded in vitro with anti-CD3/CD28 beads (Invitrogen) and interleukin-2 100 U/mL (Chiron) for up to 15 days, and transduced with lentiviral supernatant from 293T cells transfected with pELNS anti-CD123-41BB-CD3ζ plasmid DNA or with pELNS anti-CD19-41BB-CD3ζ plasmid DNA as previously reported 27 beginning on day +1 of expansion at a multiplicity of infection of 5. .. Cells The MOLM14 cell line was obtained from the ATCC and maintained in RPMI media supplemented with 10% fetal calf serum, penicillin, and streptomycin (R10).

    Isolation:

    Article Title: Personalized CRISPR Knock-In Cytokine Gene Therapy to Remodel the Tumor Microenvironment and Enhance CAR T Cell Therapy in Solid Tumors
    Article Snippet: Lymphocytes were isolated from fresh primary adult blood cells from anonymous healthy human donors using EasySep isolation kits for CD3+, CD4+ or CD8+ T cells (StemCell Technologies). .. Isolated T cells were cultured at an initial density of 10 cells per ml in X-VIVO 15 medium (Lonza) supplemented with human serum (5%, Gemini), penicillin–streptomycin (1%, Gibco), interleukin (IL-7; 5LngL/ml, Miltenyi) and IL-15 (5LngL/ml, Miltenyi). .. After isolation, cells were stimulated for 2Ldays with anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher Scientific) using a 1:1 bead-to-cell ratio.

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. The natural killer (NK) cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith and colleagues (34) and cultured in interleukin-2–supplemented (1,000 units/ mL) medium. ..

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. Cytotoxicity of natural killer (NK) and dendritic cells (DC) The NK cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith et al. ( 34 ) and cultured in interleukin-2 supplemented (1000 units/mL) media. ..

    Article Title: SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci.
    Article Snippet: Specific lymphocytes were isolated from thawed aliquots using EasySep isolation kits for CD3+, CD4+ or CD8+ T cells (StemCell Technologies). .. Isolated T cells were cultured at an initial density of 106 cells per ml in X-VIVO 15 medium (Lonza) supplemented with human serum (5%, Gemini), penicillin–streptomycin (1%, Gibco), interleukin (IL-7; 5 ng ml−1, Miltenyi) and IL-15 (5 ng ml−1, Miltenyi). .. After isolation, cells were stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher Scientific) using a 1:1 bead-to-cell ratio.

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. The NK cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith et al. ( 34 ) and cultured in interleukin-2 supplemented (1000 units/mL) media. ..

    Cell Culture:

    Article Title: Personalized CRISPR Knock-In Cytokine Gene Therapy to Remodel the Tumor Microenvironment and Enhance CAR T Cell Therapy in Solid Tumors
    Article Snippet: Lymphocytes were isolated from fresh primary adult blood cells from anonymous healthy human donors using EasySep isolation kits for CD3+, CD4+ or CD8+ T cells (StemCell Technologies). .. Isolated T cells were cultured at an initial density of 10 cells per ml in X-VIVO 15 medium (Lonza) supplemented with human serum (5%, Gemini), penicillin–streptomycin (1%, Gibco), interleukin (IL-7; 5LngL/ml, Miltenyi) and IL-15 (5LngL/ml, Miltenyi). .. After isolation, cells were stimulated for 2Ldays with anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher Scientific) using a 1:1 bead-to-cell ratio.

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. The natural killer (NK) cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith and colleagues (34) and cultured in interleukin-2–supplemented (1,000 units/ mL) medium. ..

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. Cytotoxicity of natural killer (NK) and dendritic cells (DC) The NK cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith et al. ( 34 ) and cultured in interleukin-2 supplemented (1000 units/mL) media. ..

    Article Title: SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci.
    Article Snippet: Specific lymphocytes were isolated from thawed aliquots using EasySep isolation kits for CD3+, CD4+ or CD8+ T cells (StemCell Technologies). .. Isolated T cells were cultured at an initial density of 106 cells per ml in X-VIVO 15 medium (Lonza) supplemented with human serum (5%, Gemini), penicillin–streptomycin (1%, Gibco), interleukin (IL-7; 5 ng ml−1, Miltenyi) and IL-15 (5 ng ml−1, Miltenyi). .. After isolation, cells were stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher Scientific) using a 1:1 bead-to-cell ratio.

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. The NK cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith et al. ( 34 ) and cultured in interleukin-2 supplemented (1000 units/mL) media. ..

    Control:

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. The natural killer (NK) cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith and colleagues (34) and cultured in interleukin-2–supplemented (1,000 units/ mL) medium. ..

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. Cytotoxicity of natural killer (NK) and dendritic cells (DC) The NK cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith et al. ( 34 ) and cultured in interleukin-2 supplemented (1000 units/mL) media. ..

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. The NK cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith et al. ( 34 ) and cultured in interleukin-2 supplemented (1000 units/mL) media. ..

    Magnetic Cell Separation:

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. The natural killer (NK) cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith and colleagues (34) and cultured in interleukin-2–supplemented (1,000 units/ mL) medium. ..

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. Cytotoxicity of natural killer (NK) and dendritic cells (DC) The NK cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith et al. ( 34 ) and cultured in interleukin-2 supplemented (1000 units/mL) media. ..

    Article Title: Garlic Constituent Diallyl Trisulfide Prevents Development of Poorly-Differentiated Prostate Cancer and Pulmonary Metastasis Multiplicity in TRAMP Mice
    Article Snippet: .. The NK cells were isolated from the spleen of control and DATS-treated mice using MACS beads (Myltenyi Biotech) as described by Giezeman-Smith et al. ( 34 ) and cultured in interleukin-2 supplemented (1000 units/mL) media. ..

    other:

    Article Title: SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci.
    Article Snippet: After isolation, cells were stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher Scientific) using a 1:1 bead-to-cell ratio.



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    Induction of LFs and anti-RABV-G IgG production by SAM-LNP1 in human LF chips (A) Schematic of the LF Chip created with monocytes and conditioned medium from the intramuscular vaccination-mimicking module created with BioRender.com . (B) Representative 3D confocal microscopic stacks showing pseudo-colored follicles (blue) and cell nuclei (green) present within extracellular matrix (ECM) gels cultured for 4 days within a perfused LF Chip when vaccinated with SAM-LNP1 in the absence or presence of IL-2 and IL-4 (SAM-LNP1 + IL-2&IL-4); scale bars, 100 μm. (C) Quantification of the number (left) and size (right) of LFs in LF chips of one donor based on immunostaining followed by confocal imaging. Each data point represents one field of view (left) or an individual follicle (right); 2 independent chips were analyzed per condition. (D) Anti-RABV-G IgG levels in effluents of no treatment LF chips or chips vaccinated with or without IL-2 and IL-4 and cultured for 14 days were detected using a cell-based assay; 3–4 independent chips were analyzed per condition. (E) Quantification of the number (left) and size (right) of LFs in LF chips from four different donors, with each color representing a different donor. Each data point corresponds to one field of view (left) or an individual follicle (right). (F) Anti-RABV-G IgG levels in effluents of LF chips. Each data point represents one chip, with different symbols indicating chips from three independent donors. (G) Anti-RABV-G IgG levels in effluents of LF chips seeded with naive B + bulk T + monocyte mixture. Each data point represents one chip, with different symbols indicating chips from three independent donors. (H) Heatmap showing average log2 fold changes in cytokine levels in the effluents of three LF chips created using bulk lymphocytes from donors whose prior exposure to rabies was unknown, measured using a Luminex Multiplex Assay at various time points (4, 7, 15, 22, and 28 days post-vaccination) compared to the average levels of each cytokine found at day 1. Representative results from one donor are shown in (B)–(D) and (H), with similar outcomes observed in two donors. Data shown are mean ± SD; (C, left; D; and E) one way ANOVA test followed by Tukey’s multiple comparisons test, (C, right; and E) Mann-Whitney U test, (F and G) Welch’s t test.

    Journal: iScience

    Article Title: In vitro recapitulation of intramuscular mRNA vaccination with naive and recall antigens using a human lymphoid follicle chip platform

    doi: 10.1016/j.isci.2026.116416

    Figure Lengend Snippet: Induction of LFs and anti-RABV-G IgG production by SAM-LNP1 in human LF chips (A) Schematic of the LF Chip created with monocytes and conditioned medium from the intramuscular vaccination-mimicking module created with BioRender.com . (B) Representative 3D confocal microscopic stacks showing pseudo-colored follicles (blue) and cell nuclei (green) present within extracellular matrix (ECM) gels cultured for 4 days within a perfused LF Chip when vaccinated with SAM-LNP1 in the absence or presence of IL-2 and IL-4 (SAM-LNP1 + IL-2&IL-4); scale bars, 100 μm. (C) Quantification of the number (left) and size (right) of LFs in LF chips of one donor based on immunostaining followed by confocal imaging. Each data point represents one field of view (left) or an individual follicle (right); 2 independent chips were analyzed per condition. (D) Anti-RABV-G IgG levels in effluents of no treatment LF chips or chips vaccinated with or without IL-2 and IL-4 and cultured for 14 days were detected using a cell-based assay; 3–4 independent chips were analyzed per condition. (E) Quantification of the number (left) and size (right) of LFs in LF chips from four different donors, with each color representing a different donor. Each data point corresponds to one field of view (left) or an individual follicle (right). (F) Anti-RABV-G IgG levels in effluents of LF chips. Each data point represents one chip, with different symbols indicating chips from three independent donors. (G) Anti-RABV-G IgG levels in effluents of LF chips seeded with naive B + bulk T + monocyte mixture. Each data point represents one chip, with different symbols indicating chips from three independent donors. (H) Heatmap showing average log2 fold changes in cytokine levels in the effluents of three LF chips created using bulk lymphocytes from donors whose prior exposure to rabies was unknown, measured using a Luminex Multiplex Assay at various time points (4, 7, 15, 22, and 28 days post-vaccination) compared to the average levels of each cytokine found at day 1. Representative results from one donor are shown in (B)–(D) and (H), with similar outcomes observed in two donors. Data shown are mean ± SD; (C, left; D; and E) one way ANOVA test followed by Tukey’s multiple comparisons test, (C, right; and E) Mann-Whitney U test, (F and G) Welch’s t test.

    Article Snippet: Human Interleukin-4 (IL-4) , Miltenyi Biotec , 130-093-922.

    Techniques: Cell Culture, Immunostaining, Imaging, Cell Based Assay, Luminex, Multiplex Assay, MANN-WHITNEY